6 . oocytes were fixed for immunofluorescence . examination of cgs and microtubules were performed by fitc labeled lens culinaris agglutinin ( lca ) and and - fi - tubulin under confocal scanning laser microscopy ( cslm ) respectively 利用直接免疫荧光染色和共聚焦显微镜（ confocalscan muglasermicroscopy ， cslm ） ，研究各组体夕成熟卵的皮质颗粒和微管
